The function of RPUSD1 (RNA pseudouridine synthase domain containing 1, Ensembl gene identifier ENSG00000007376) is as follows. Pseudouridylate synthase that catalyzes the conversion of uridine to pseudouridine in cytoplasmic tRNAs. Catalyzes pseudouridylation of pseudouridine-30 and pseudouridine-72 from uracil- 30 and uracil-72 within the anticodon stem of cytoplasmic tRNAs. .