The function of Ensembl gene identifier ENSG00000043514 (TRIT1, tRNA isopentenyltransferase 1) is as follows. Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i6A37) (PubMed:11111046, PubMed:24126054, PubMed:24901367, PubMed:34774131). Mediates modification of a limited subset of tRNAs: tRNA(Ser)(AGA), tRNA(Ser)(CGA), tRNA(Ser)(UGA), as well as partial modification of the selenocysteine tRNA(Ser)(UCA) (PubMed:24126054). TRIT1 is therefore required for selenoprotein expression (PubMed:24126054). {ECO:0000269|PubMed:11111046, ECO:0000269|PubMed:24126054, ECO:0000269|PubMed:24901367, ECO:0000269|PubMed:34774131}.