The function of KIF25 (kinesin family member 25, Ensembl gene identifier ENSG00000125337) is as follows. Minus-end microtubule-dependent motor protein (By similarity). Acts as a negative regulator of centrosome separation required to prevent premature centrosome separation during interphase (PubMed:28263957). Required to maintain a centered nucleus to ensure that the spindle is stably oriented at the onset of mitosis (PubMed:28263957). May also act as a negative regulator of amino acid starvation-induced autophagy (PubMed:22354037). {ECO:0000250|UniProtKB:Q4R918, ECO:0000269|PubMed:22354037, ECO:0000269|PubMed:28263957}.