The function of TSLIG2 (tRNA splicing ligase complex subunit 2, Ensembl gene identifier ENSG00000135974) is as follows. Accessory subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity and may function toward other RNAs. .