Peroxisomal protease that mediates both the removal of the leader peptide from proteins containing a PTS2 target sequence and processes several PTS1-containing proteins. Catalyzes the processing of PTS1-proteins involved in the peroxisomal beta-oxidation of fatty acids. . This is the function of Ensembl gene identifier ENSG00000156521 (TYSND1, trypsin like peroxisomal matrix peptidase 1).