The function of PARS2 (prolyl-tRNA synthetase 2, mitochondrial, Ensembl gene identifier ENSG00000162396) is as follows. Mitochondrial aminoacyl-tRNA synthetase that catalyzes the specific attachment of the proline amino acid (aa) to the homologous transfer RNA (tRNA), further participating in protein synthesis. The reaction occurs in a two steps: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). .