The function of DTWD2 (DTW motif tRNA-uridine aminocarboxypropyltransferase 2, ENSG00000169570) is as follows. Catalyzes the formation of 3-(3-amino-3-carboxypropyl)uridine (acp3U) at position 20a in the D-loop of several cytoplasmic tRNAs (acp3U(20a)) (PubMed:31804502, PubMed:39173631). Also has a weak activity to form acp3U at position 20 in the D-loop of tRNAs (acp3U(20)) (PubMed:31804502). Involved in glycoRNA biosynthesis by mediating formation of acp3U, which acts as an attachment site for N- glycans on tRNAs (PubMed:39173631). GlycoRNAs consist of RNAs modified with secretory N-glycans that are presented on the cell surface (PubMed:39173631). {ECO:0000269|PubMed:31804502, ECO:0000269|PubMed:39173631}.