The function of Ensembl gene identifier ENSG00000178694 (NSUN3, NOP2/Sun RNA methyltransferase 3) is as follows. Mitochondrial tRNA methyltransferase that mediates methylation of cytosine to 5-methylcytosine (m5C) at position 34 of mt- tRNA(Met) (PubMed:27214402, PubMed:27356879, PubMed:27497299). mt- tRNA(Met) methylation at cytosine(34) takes place at the wobble position of the anticodon and initiates the formation of 5- formylcytosine (f(5)c) at this position (PubMed:27214402, PubMed:27356879, PubMed:27497299). mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation (PubMed:27356879, PubMed:27497299). {ECO:0000269|PubMed:27214402, ECO:0000269|PubMed:27356879, ECO:0000269|PubMed:27497299}.